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| Registros recuperados: 66 | |
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| Özşensoy,Yusuf. |
| ABSTRACT The objective of this study was to investigate polymorphisms both in exons 1 and 3 of prolactin (PRL) gene for milk productivity of Anatolian water buffalo breed in Sivas province in Turkey. Blood samples were collected from 129 male and female water buffaloes and DNA was isolated by using phenol/chloroform method. Samples of DNA were amplified and resulting PCR products were digested with RsaI (for exons 1 and 3) and HaeIII (for exon 1). Allelic polymorphisms were determined by separation of fragments obtained from digested PCR products in 3% agarose gel electrophoresis. AA genotype (HaeIII) and BB genotype (RsaI) of exon 1 and only AA genotype (RsaI) of exon 3 were obtained. No polymorphisms were determined in Anatolian water buffalo breed and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HaeIII; PCR-RFLP; Prolactin; RsaI; Sivas. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100403 |
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| Otaviano,Antonio Roberto; Tonhati,Humberto; Sena,Janete Aparecida Desidério; Cerón Muñoz,Mario Fernando. |
| Caseins comprise make up about 80% of the total protein content of milk and present polymorphism with changes in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Buffaloes; Polymorphism; PCR-RFLP; SSCP. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200010 |
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| He,H; Wang,Y; Qing,Y; Li,D; Zhao,X; Zhu,Q; Yin,H. |
| ABSTRACT In this study, a method utilizing PCR-restriction fragment length polymorphism (PCR-RFLP) of a mitochondrial gene was developed for the identification of chicken (Gallus gallus), quail (Coturnix coturnix), and common pigeon (Columba livia) meat. PCR products of ~440 bp were obtained from the 12S rRNA gene of these three birds using a pair of universal primers. The three terrestrial birds can be distinguished using one restriction endonuclease, Alu I, which was selected based on species-specific variations in the mt 12S rRNA gene sequence using 9 newly-obtained and 44 published chicken, quail and pigeon sequences. This method was also successfully used to identify commercial quail and pigeon meat products, which were found to be adulterated with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 12S rRNA gene; PCR-RFLP; Terrestrial bird; Meat identification; Species-specific variations. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400651 |
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| Gibotti,Adriana; Tanaka,Tânia L.; Oliveira,Valéria R.; Taddei,Carla R.; Martinez,Marina B.. |
| In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ipa; Enteroinvasive Escherichia coli; Polymorphism; PCR-RFLP. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012 |
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| Almeida,Álvaro M. R.; Piuga,Fernanda F.; Marin,Silvana R. R.; Binneck,Eliseu; Sartori,Fábio; Costamilan,Leila M.; Teixeira,Maria R. O.; Lopes,Marcelo. |
| Cercospora kikuchii, involved with the defoliation of soybean (Glycine max) plants, is normally associated with Septoria glycines in late season. Seventy-two isolates from different regions in Brazil, obtained mainly from purple stained seeds, showed phenotypic variation. Cercosporin content and rate of colony growth was higly variable among isolates. A strong correlation between cercosporin content and virulence was identified. Genetic variation among and within populations was evaluated based on 86 RAPD loci. The RAPD analysis clustered all isolates into seven groups. No relationship was observed between RAPD groups and geographic origin or cercosporin content. The sequence of the internal spacer regions (ITS1-5.8S-ITS2) from 13 isolates chosen according... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotypic diversity; PCR-RFLP; RAPD; ITS sequence; Virulence. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000600005 |
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| Lelievre, Stephanie; Verrez-bagnis, Veronique; Jerome, Marc; Vaz, Sandrine. |
| A better knowledge on spawning grounds of principal commercial fishes from North Sea is necessary for their monitoring. Fish egg taxonomic identification based on morphological characters cannot always be carried out effectively. This is particularly the case for a number of Gadidae fish species eggs (i.e. cod, haddock and whiting, and three rockling species) which have the same range of size and for which molecular-based techniques can help improving the accuracy of egg taxonomic identification, and hence the reliability of studies on spawning areas. In this study, formalin fixation, egg conservation and DNA extraction were optimized in order to develop a specific PCR-RFLP method. Based on the sequence of the cytochrome b gene of reference specimens, a... |
| Tipo: Text |
Palavras-chave: Fish eggs; PCR-RFLP; Cytochrome b gene; Geostatistical analyses; Interpolated map. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00005/11613/8259.pdf |
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| Golfetto,L.; Alves,E.V.; Martins,T.R.; Sincero,T.C.M.; Castro,J.B.S.; Dannebrock,C.; Oliveira,J.G.; Levi,J.E.; Onofre,A.S.C.; Bazzo,M.L.. |
| Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology and another sample was collected using BD SurePath™ kit for HPV tests. Eighty samples (24.6%) were positive for HPV gene by PCR-Multiplex and were then submitted to PCR-RFLP and PapilloCheck® microarray. There was a genotyping agreement in 71.25% (57/80) on at least one HPV type between PCR-RFLP and PapilloCheck® microarray. In 22 samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cervical cancer; HPV; Screening; PCR-RFLP; PapilloCheck® microarray. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500619 |
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| Blanck,Danielly Veloso; Gasparino,Eliane; Ribeiro,Ricardo Pereira; Marques,Débora Sommer. |
| O objetivo deste trabalho foi determinar a associação de polimorfismos no gene do hormônio crescimento GH1 às características corporais, em linhagens de tilápia-do-nilo (Oreochromis niloticus). Foram coletados fragmentos da nadadeira caudal de exemplares das linhagens, aos cinco meses de idade, para as análises de "polymerase chain reaction-restriction fragment lenght polymorphism" (PCR-RFLP). Foram realizadas as seguintes mensurações: comprimento total, comprimento padrão, altura, largura e comprimento da cabeça. Realizou-se a amplificação de um fragmento com 652 pb do gene GH1, com subsequente restrição com a enzima PstI. Para a análise de associação do marcador molecular com as características quantitativas, utilizou-se o procedimento GLM do SAS. O... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oreochromis niloticus; Enzima de restrição; Hormônio do crescimento; PCR-RFLP. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000600008 |
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| Gomes,Eliane A.; Kasuya,Maria Catarina M.; Barros,Everaldo G. de; Borges,Arnaldo C.; Araújo,Elza F.. |
| Inter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA fingerprinting; Ectomycorrhizal fungi; Internal transcribed spacer (ITS); PCR-RFLP; Ribosomal DNA (rDNA). |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400018 |
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| Fernández,Ilda G.; Ramírez,José Gonzalo Ríos; Vázquez,Amanda Gayosso; Arvizu,Raúl Ulloa; Morales,Rogelio A. Alonso. |
| The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916,... |
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Palavras-chave: BoLA-DRB3.2; Polymorphism; Creole cattle; PCR-RFLP. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500013 |
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| Zhan,Xiaoli; Jiang,Bijie; Ma,Xianghui; Fu,Changzhen; Zan,Linsen. |
| Background: We investigated the polymorphisms of the bovine chemokine receptor-like 1(CMKLR1) gene. The coding region of CMKLR1 was screened in Qinchuan cattle by PCR-RFLP technology. Results: In this study, we discovered two single nucleotide polymorphisms (SNPs) (264G > C and 762C > T) in the coding region of the CMKLR1 gene. Hence, we described the BmgT120l and Pdm1 PCR-RFLP methods for detecting the 64G > C and 762C > T mutations, respectively. PCR-RFLP and sequencing were used to analyze the two loci of CMKLR1 gene in 324 individuals, which were randomly selected from breeding populations. Furthermore, meat quality traits in another 80 Qinchuan individuals were analyzed by the comparison between the genotypes and their phenotypic data.... |
| Tipo: Journal article |
Palavras-chave: CMKLR1 gene; Meat quality traits; PCR-RFLP; Qinchuan cattle; SNP. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100003 |
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| Ozbey,Gokben; Dogan,Yasar; Demiroren,Kaan; Ozercan,Ibrahim Hanifi. |
| The objectives of the present study were to determine Helicobacter pylori via culture, polymerase chain reaction and histopathological diagnosis in 101 children ranging in age from 4 to 18 years, to identify the association among restriction fragment length polymorphism types and clinical disease and to investigate the relationships among different isolates of H. pylori in different age groups. We observed a high prevalence of H. pylori infections in children between the ages of 13 and 18 (75.8%), while children aged 4 to 6 years had the lowest prevalence of infection (40%). H. pylori was detected in 30.7% (31 of 101), 66.3% (67 of 101) and 63.2% (60 of 95) of children as determined by culture methods, PCR and histological examination, respectively. H.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Children; Culture; Helicobacter pylori; PCR-RFLP. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200505 |
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| Zarrinfar,Hossein; Kaboli,Saeed; Dolatabadi,Somayeh; Mohammadi,Rasoul. |
| Abstract Candida species, especially C. albicans, are commensals on human mucosal surfaces, but are increasingly becoming one of the important invasive pathogens as seen by a rise in its prevalence in immunocompromised patients and in antibiotic consumption. Thus, an accurate identification of Candida species in patients with pulmonary symptoms can provide important information for effective treatment. A total of 75 clinical isolates of Candida species were obtained from the bronchoalveolar lavage fluid of both immunocompromised and immunocompetent patients with pulmonary symptoms. Candida cultures were identified based on nuclear ribosomal Internal Transcribed Spacer (ITS1-ITS2 rDNA) sequence analysis by polymerase chain reaction–restriction fragment... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida; Pulmonary; PCR-RFLP. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100172 |
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| Band,Guilherme de Oliveira; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Peixoto,Jane de Oliveira; Faria,Danielle Assis; Pires,Aldrin Vieira; Figueiredo,Frederico de Castro; Nascimento,Carlos Souza do; Gomide,Lúcio Alberto de Miranda. |
| The PSS genotypes of 596 F2 pigs produced by initial mating of Brazilian native boars commercial sows and were characterized by PCR-RFLP and their carcass and performance traits were evaluated. Among the 596 animals analyzed, 493 (82.72%) were characterized as NN and 103 (17.28%) as Nn. With respect to carcass traits, Nn animals presented higher (p < 0.05) right half carcass weight, left half carcass weight, loin depth and loin eye area, and lower shoulder backfat thickness, backfat thickness between last and next to last but one lumbar vertebrae and backfat thickness after last rib at 6.5 cm from the midline compared to NN animals. Nn animals also showed (p < 0.05) higher values for most of the cut yields, indicating higher cutting yields for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carcass; Performance; PSS gene; PCR-RFLP; Pig. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100016 |
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| Band,Guilherme de Oliveira; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Schierholt,Alex Sandro; Silva,Kleibe Moraes; Pires,Aldrin Vieira; Benevenuto Júnior,Augusto Aloísio; Gomide,Lúcio Alberto de Miranda. |
| The PSS genotypes of 596 F2 pigs produced by initial mating of Brazilian commercial sows and native boars were characterized by PCR-RFLP and the pork quality traits were evaluated. Among the 596 pigs studied, 493 (82.7%) were NN and 103 (17.3%) were Nn. There were no differences between NN and Nn pigs in the following pork qualities: pHu (5.71 ± 0.16 vs 5.70 ± 0.11), intramuscular fat (1.55 ± 0.64% vs 1.65 ± 0.67%), shear force (5552 ± 878 g/1.2 cm vs 5507 ± 826 g/1.2 cm), lightness (44.96 ± 2.05 vs 45.01 ± 1.92), redness (0.64 ± 0.60 vs 0.79 ± 0.55), yellowness (6.62 ± 0.56 vs 6.65 ± 0.48), hue (84.28 ± 5.53 vs 83.41 ± 4.85), or chroma (6.68 ± 0.52 vs 6.73 ± 0.52). However, pork from Nn pigs had a significantly (p < 0.05) lower pH45 (6.41 ± 0.27 vs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meat quality; PCR-RFLP; Pork; PSE; PSS; Pig. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100015 |
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| Faure, Stephanie. |
| La marteiliose est une maladie causée par Marteilia refringens chez l'huître plate, Ostrea edulis. Ce parasite induit des mortalités importantes chez ces mollusques bivalves. Une autre espèce, Marteilia maurini, parasite la moule, Mytilus edulis, sans engendrer de mortalités notables. Des cas de coinfections des moules par ces 2 parasites ont été observés. La moule pourrait donc être vecteur de Marteilia refringens. Des études sur la taxinomie de Marteilia refringens ont démontré qu'il existe un polymorphisme entre les 2 espèces dans l'ITS1. De ce fait, une technique de PCR-RFLP a été mise au point pour discriminer les 2 espèces. Le but de cette étude est d'éclaircir le statut de la moule dans le cycle biologique de Marteilia refringens par deux approches... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Marteilia maurini; Mytilus edulis; Vecteur; PCR-RFLP; PCR; Pathologie. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00032/14343/11627.pdf |
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| Mathia, Olivier. |
| Marteilia refringens est l'un des parasites de l'huître plate, Ostrea edulis ; la marteiliose dont il est l'agent causal provoque une forte mortalité chez les mollusques bivalves. Le genre Marteilia comprend d'autres espèces telle que Marteilia maurini qui lui affecte la moule, Mytilus edulis. Il a été démontré, dans quelques rares cas que les deux espèces pouvaient co habiter au sein d'une même moule. Ainsi le mollusque pourrait être vecteur de Marteilia refringens. Certaines études ont montré par PCR et PCR-RFLP qu'il existait un dimorphisme dans la région ITS-1 des gènes ribosomaux chez Marteilia. Ces techniques m'ont donc permis d'observer la prévalence du phénomène de co-detection dans le milieu naturel et d'étudier l'implication de la moule dans le... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Marteilia maurini; Mytilus edulis; Vecteur; PCR; PCR-RFLP. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00369/47981/48007.pdf |
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| Registros recuperados: 66 | |
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